glcK [2019-08-07 12:35:26]

glucose kinase (D-glucose:ATP)

Locus: BSU_24850

Isoelectric point: 5.99

Molecular weight: 33.39 kDa

Protein length: 321 aa

Gene length: 966 bp

Function: phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides

Product: glucose kinase (D-glucose:ATP)

Essential: no

E.C.: 2.7.1.2

Synonyms: yqgR

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of trehalose
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of maltose
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of starch/ maltodextrin

Gene

Coordinates: 2,570,606 2,571,571

The protein

Catalyzed reaction/ biological activity

• ATP + D-glucose = ADP + D-glucose 6-phosphate (according to Swiss-Prot)

Protein family

• ROK (NagC/XylR) family (with GmuE and XylR, according to UniProt)

Paralogous protein(s)

• XylR

Structure

• 2QM1 (from Enterococcus faecalis, 38% identity, 52% similarity)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: yqgP-yqgO-glcK-yqgS
  Description: PubMed

  Additional information

  • PhoP∼P binds to the promoter region, but regulation has not been explored PubMed

Biological materials

Mutant

• GP2 (spc), available in Jörg Stülke's lab
• BKE24850 (glcK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT
• BKK24850 (glcK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT

References

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